Question 1

An effective tool for screening a large number of genetic sequences at once is known as

Accepted Answer

A)   restriction analysis.
B)   microarray.
C)   FISH.
D)   gel electrophoresis.
E)   cDNA synthesis.F) C) and E)
G) A) and E)

Question 2

Sticky- end fragments generated by EcoRI will hydrogen bond to any other sticky- end sequence.

Accepted Answer

A) True 
 B)False

Question 3

The insertion of foreign DNA directly into a cell's nucleus using a glass micropipet is called (microporation/electroporation/microinjection).

Accepted Answer

microinjection

Question 4

Two men may be the father of a child. This figure shows the results of a DNA fingerprint analysis to determine paternity. M is the mother, C is the child, A and B are the two men. What is the best interpretation of these results?

Accepted Answer

A)   Neither man is this child's father.
B)   The man identified as A is probably the father.
C)   Either man could be this child's father.
D)   The man identified as B is probably the father.
E)   Paternity cannot be determined from this data.F) A) and D)
G) A) and C)

Question 5

Genetic (screening/sequencing/cloning) can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Accepted Answer

The answer of Genetic (screening/sequencing/cloning) can be used to detect...

Question 6

Discuss one of the pros and cons regarding the application of recombinant DNA technology.

Accepted Answer

One of the pros of the application of re...

Question 7

Outline a procedure for producing a useful new recombinant product.

Accepted Answer

Many variations are possible. The answer...

Question 8

Which of the following is NOT a goal of recombinant DNA technology?

Accepted Answer

A)   making it possible to clone humans
B)   being able to remove or correct damaging traits in humans
C)   combining genetic material from more than one organism to produce new useful organisms
D)   creating organisms capable of producing useful products
E)   eliminating undesirable traits from livestock or cropsF) B) and D)
G) C) and E)

Question 9

Restriction enzymes are useful only on synthetic DNAs.

Accepted Answer

A) True 
 B)False

Question 10

The goals of recombinant DNA technology include production of new organisms with useful combinations of traits.

Accepted Answer

A) True 
 B)False  True

Question 11

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following restriction enzyme sites would produce blunt- ended fragments? (The arrow represents the cutting site of the enzyme.)

Accepted Answer

A)   CCC$GGG
B)   G$AATTC
C)   C$CGG
D)   A$AGCTT
E)   G$GATCCF) B) and D)
G) A) and E)

Question 13

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Accepted Answer

A)   DNA replication would stop after one cycle.
B)   DNA replication would occur more slowly than normal.
C)   DNA replication would occur twice as fast as normal.
D)   DNA replication would not occur at all.
E)   Many mistakes would occur.F) All of the above
G) B) and E)

Question 14

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Accepted Answer

A)   Southern blotting
B)   gene gun
C)   electroporation
D)   protoplast fusion
E)   microinjectionF) A) and D)
G) C) and D)

Question 15

Which of the following items is NOT a part of the name of a restriction enzyme?

Accepted Answer

A)   the strain of the source bacterium
B)   the specific epithet of the source bacterium
C)   the genus of the source bacterium
D)   Roman numerals to indicate its order of discovery
E)   the Gram reaction of the source bacteriumF) A) and E)
G) B) and E)

Question 16

A library of cloned sequences representing the expressed genes of an organism is known as a

Accepted Answer

A)   microarray.
B)   cDNA library.
C)   DNA fingerprint.
D)   FISH library.
E)   gene library.F) B) and E)
G) A) and C)

Question 17

Explain what a transgenic organism is, and give two examples.

Accepted Answer

A transgenic organism is one that contains genes from other organisms. These genes usually add some special ability or function to the organism. Agriculture offers many examples of transgenic organisms. Soybeans have been engineered to contain glyphosate- resistance genes, and other crops have been engineered to grow well in soil with high salt concentrations. Plants have been modified to be resistant to naturally occurring microbial diseases. Still other plants have been engineered to contain the gene for Bt toxin, a bacterial toxin that kills insect pests that would otherwise feed on and destroy the crop. Not all transgenic organisms represent such extreme genetic modifications, however. Some genetically modified organisms simply represent efforts to improve nutritional content (in the case of plant crops) or to increase food yield (in the case of bovine growth hormone, which is produced by transgenic bacteria and then given to cattle to increase their meat and milk yield).

Question 18

The sequencing and analysis of an organism's genetic information is called

Accepted Answer

A)   gene therapy.
B)   genomics.
C)   northern blotting.
D)   PCR.
E)   protein synthesis.F) C) and D)
G) All of the above

Question 19

are used for cutting DNA molecules into fragments.

Accepted Answer

A)   Antisense RNAs
B)   RNA polymerases
C)   Mutagens
D)   DNA polymerase and DNA ligase
E)   Restriction enzymesF) A) and B)
G) B) and E)

Question 20

Small RNAs that bind to an mRNA and alter its expression are known as (antisense/probes/restriction) RNAs.

Accepted Answer

The answer of Small RNAs that bind to an mRNA...

Exam 8: Recombinant Dna Technology

An effective tool for screening a large number of genetic sequences at once is known as

Correct Answer
verified

Sticky- end fragments generated by EcoRI will hydrogen bond to any other sticky- end sequence.

Correct Answer
verified

The insertion of foreign DNA directly into a cell's nucleus using a glass micropipet is called (microporation/electroporation/microinjection).

Correct Answer
verified
microinjection

Two men may be the father of a child. This figure shows the results of a DNA fingerprint analysis to determine paternity. M is the mother, C is the child, A and B are the two men. What is the best interpretation of these results?

Correct Answer
verified

Genetic (screening/sequencing/cloning) can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Correct Answer
verified

Discuss one of the pros and cons regarding the application of recombinant DNA technology.

Correct Answer
Answered by ExamLex AI
One of the pros of the application of re...
View Answer

Outline a procedure for producing a useful new recombinant product.

Correct Answer
verified
Many variations are possible. The answer...
View Answer

Which of the following is NOT a goal of recombinant DNA technology?

Correct Answer
verified

Restriction enzymes are useful only on synthetic DNAs.

Correct Answer
verified

The goals of recombinant DNA technology include production of new organisms with useful combinations of traits.

Correct Answer
verified
True

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen.

Correct Answer
verified

Which of the following restriction enzyme sites would produce blunt- ended fragments? (The arrow represents the cutting site of the enzyme.)

Correct Answer
verified

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Correct Answer
verified

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Correct Answer
verified

Which of the following items is NOT a part of the name of a restriction enzyme?

Correct Answer
verified

A library of cloned sequences representing the expressed genes of an organism is known as a

Correct Answer
verified

Explain what a transgenic organism is, and give two examples.

The sequencing and analysis of an organism's genetic information is called

Correct Answer
verified

are used for cutting DNA molecules into fragments.

Correct Answer
verified

Small RNAs that bind to an mRNA and alter its expression are known as (antisense/probes/restriction) RNAs.

Correct Answer
verified

Exam 8: Recombinant Dna Technology

An effective tool for screening a large number of genetic sequences at once is known as

Correct AnswerverifiedShow Answer

Sticky- end fragments generated by EcoRI will hydrogen bond to any other sticky- end sequence.

Correct AnswerverifiedShow Answer

The insertion of foreign DNA directly into a cell's nucleus using a glass micropipet is called (microporation/electroporation/microinjection).

Correct Answerverifiedmicroinjection

Two men may be the father of a child. This figure shows the results of a DNA fingerprint analysis to determine paternity. M is the mother, C is the child, A and B are the two men. What is the best interpretation of these results?

Correct AnswerverifiedShow Answer

Genetic (screening/sequencing/cloning) can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Correct AnswerverifiedShow Answer

Discuss one of the pros and cons regarding the application of recombinant DNA technology.

Correct AnswerAnswered by ExamLex AIOne of the pros of the application of re...View AnswerShow Answer

Outline a procedure for producing a useful new recombinant product.

Correct AnswerverifiedMany variations are possible. The answer...View AnswerShow Answer

Which of the following is NOT a goal of recombinant DNA technology?

Correct AnswerverifiedShow Answer

Restriction enzymes are useful only on synthetic DNAs.

Correct AnswerverifiedShow Answer

The goals of recombinant DNA technology include production of new organisms with useful combinations of traits.

Correct AnswerverifiedTrue

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen.

Correct AnswerverifiedShow Answer

Which of the following restriction enzyme sites would produce blunt- ended fragments? (The arrow represents the cutting site of the enzyme.)

Correct AnswerverifiedShow Answer

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Correct AnswerverifiedShow Answer

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Correct AnswerverifiedShow Answer

Which of the following items is NOT a part of the name of a restriction enzyme?

Correct AnswerverifiedShow Answer

A library of cloned sequences representing the expressed genes of an organism is known as a

Correct AnswerverifiedShow Answer

Explain what a transgenic organism is, and give two examples.

The sequencing and analysis of an organism's genetic information is called

Correct AnswerverifiedShow Answer

are used for cutting DNA molecules into fragments.

Correct AnswerverifiedShow Answer

Small RNAs that bind to an mRNA and alter its expression are known as (antisense/probes/restriction) RNAs.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
microinjection

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
One of the pros of the application of re...
View Answer

Correct Answer
verified
Many variations are possible. The answer...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
True

Correct Answer
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Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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